αvβ3 integrin-dependent endothelial cell dynamics in vivo

Paul A. Rupp, A. Czirók, Charles D. Little

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A major challenge confronting developmental cell biologists is to understand how individual cell behaviors lead to global tissue organization. Taking advantage of an endothelial cell-specific marker and scanning time-lapse microscopy, we have examined the formation of the primary vascular pattern during avian vasculogenesis. Five types of distinguishable endothelial cell motion are observed during formation of a vascular plexus: (1) global tissue deformations that passively convect endothelial cells; (2) vascular drift, a sheet-like medial translocation of the entire vascular plexus; (3) structural rearrangements, such as vascular fusion; (4) individual cell migration along existing endothelial structures; and (5) cell process extension into avascular areas, resulting in new links within the plexus. The last four types of motion are quantified and found to be reduced in the presence of an α vβ3 integrin inhibitor. These dynamic cell motility data result in new hypotheses regarding primordial endothelial cell behavior during embryonic vasculogenesis.

Original languageEnglish
Pages (from-to)2887-2897
Number of pages11
JournalDevelopment
Volume131
Issue number12
DOIs
Publication statusPublished - Jun 2004

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Integrins
Blood Vessels
Endothelial Cells
Cell Movement
Microscopy

Keywords

  • αvβ3 integrin
  • Computational biology
  • Endothelial cells
  • Quail
  • Time-lapse
  • Vasculogenesis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

αvβ3 integrin-dependent endothelial cell dynamics in vivo. / Rupp, Paul A.; Czirók, A.; Little, Charles D.

In: Development, Vol. 131, No. 12, 06.2004, p. 2887-2897.

Research output: Contribution to journalArticle

Rupp, Paul A. ; Czirók, A. ; Little, Charles D. / αvβ3 integrin-dependent endothelial cell dynamics in vivo. In: Development. 2004 ; Vol. 131, No. 12. pp. 2887-2897.
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