α-Chymotrypsin-catalysed synthesis of N-acetyl-l-tyrosine esters in organic media

K. László, L. M. Simon

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

α-Chymotrypsin was used to catalyse the esterification of N-acetyl-l-tyrosine with primary alcohols in different water-miscible (acetone, 1,4-dioxane, acetonitrile, methanol, ethanol, tetrahydrofuran, N,N-dimethylformamide, dimethyl sulfoxide) and water-immiscible (toluene, ethyl acetate, diethyl ether, chloroform, carbon tetrachloride) organic solvents. Acetone proved to be the most efficient solvent and the highest yield of ester was produced with methanol. The formation of N-acetyl-l-Tyr methyl ester with ga-chymotrypsin in acetone was studied in detail and optimized. The conversion to the methyl ester was the highest (83.9%) when 2.17 mg/ml N-acetyl-l-Tyr was incubated with 10% methanol and 2 mg/ml α-chymotrypsin in acetone with a water content of about 3% at pH 7.0 and 30 °C for 24 h.

Original languageEnglish
Title of host publicationProgress in Biotechnology
PublisherElsevier
Pages713-718
Number of pages6
EditionC
DOIs
Publication statusPublished - 1998

Publication series

NameProgress in Biotechnology
NumberC
Volume15
ISSN (Print)0921-0423

ASJC Scopus subject areas

  • Biotechnology

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    László, K., & Simon, L. M. (1998). α-Chymotrypsin-catalysed synthesis of N-acetyl-l-tyrosine esters in organic media. In Progress in Biotechnology (C ed., pp. 713-718). (Progress in Biotechnology; Vol. 15, No. C). Elsevier. https://doi.org/10.1016/S0921-0423(98)80108-1