Az euphrasia rostkoviana hayne fenoloid tartalmú frakcióinak antioxidÁns értékelése

Translated title of the contribution: Á.: Antioxidant activity of different phenolic fractions separated from Euphrasia rostkoviana Hayne

Balázs Blazics, Ágnes Alberti, A. Kéry

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Euphrasia rostkoviana Hayne (Eyebright) is a valuable part of the traditional folk medicine as an anti-inflammatory herb since centuries. Since oxidative stress may underlie as a key feature of inflammatory process, the methanolic extract of Euphrasia rostkoviana Hayne (eyebright) and its methanolic fractions were evaluated for antioxidant activity by the use of ABTS and DPPH de- colorization assays. Fractionation of the Soxhlet extract was accomplished by polyamide column chromatography. For chemical characterisation HPLC-DAD-MS/MS was used. Fraction 1., dominated by a glycosilated caffeic acid derivative, exhibited the strongest antioxidant activity in DPPH and ABTS assays, IC 50: 11.88 μg/ml and 4.24 μg/ml, respectively, which are notable results if compared to the investigated standards. Fractions of flavonoid glycosides proved to have similarly strong, but lower effect. The scavenging reaction of the two radical cations were comparable, IC 50 results were differing, while precision not.

Original languageHungarian
Pages (from-to)11-16
Number of pages6
JournalActa Pharmaceutica Hungarica
Volume79
Issue number1
Publication statusPublished - 2009

Fingerprint

Euphrasia
Antioxidants
Traditional Medicine
Nylons
Glycosides
Flavonoids
Cations
Chromatography
Oxidative Stress
Anti-Inflammatory Agents
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Az euphrasia rostkoviana hayne fenoloid tartalmú frakcióinak antioxidÁns értékelése. / Blazics, Balázs; Alberti, Ágnes; Kéry, A.

In: Acta Pharmaceutica Hungarica, Vol. 79, No. 1, 2009, p. 11-16.

Research output: Contribution to journalArticle

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